Part:BBa_K3255001:Design

Transcriptional unit for an enzyme of the athocyanine route synthesis

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 68
  Illegal PstI site found at 127
  Illegal PstI site found at 439
  Illegal PstI site found at 784
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  Illegal SpeI site found at 68
  Illegal PstI site found at 127
  Illegal PstI site found at 439
  Illegal PstI site found at 784
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 68
  Illegal PstI site found at 127
  Illegal PstI site found at 439
  Illegal PstI site found at 784
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 68
  Illegal PstI site found at 127
  Illegal PstI site found at 439
  Illegal PstI site found at 784
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

.

Source

.The coding gene has a plant origin, from Camelia sinensis

References

. J. A. Jones, V. R. Vernacchio, S. M. Collins, A. N. Shirk, Y. Xiu, J. A. Englaender, B. F. Cress, C. C. McCutcheon, R. J. Linhardt, R. A. Gross, M. A. G. Koffas. Complete Biosynthesis of Anthocyanins Using E. coli Polycultures. mBio 8 (2017) e00621-17.

J. Zha, M. A.G. Koffas. Production of anthocyanins in metabolically engineered microorganisms: Current status and perspectives. Synthetic and Systems Biotechnology 2 (2017) 259-266.

I. Appelhagen, A. K. Wulff-Vester, M. Wendell, A.-K. Hvoslef-Eide, J. Russell, A. Oertel, S. Martens, H.-P. Mock, C. Martin, A. Matros. Colour bio-factories: Towards scale-up production of anthocyanins in plant cell cultures. Metabolic Engineering 48 (2018) 218-232.

B. Shrestha, R. P. Pandey, S. Darsandhari, P. Parajuli, J. K. Sohng. Combinatorial approach for improved cyanidin 3‑O‑glucoside production in Escherichia coli. Microb Cell Fact 18 (2019) 7.